Chemistry Notes for B.Sc. and M.Sc.

In flame Emission Spectrometry, the combustion flame not only frees the atoms but it also supplies the energy necessary to move the electrons of the free atoms from ground state to excited state.  The energy which is emitted by the excited atoms when returning to the ground state provides the basis for analytical determination in Flame Emission Spectrometry. Applications of Atomic Emission Spectrometry: 1. Food Industry: for detection of calcium and iron in beer . 2. Biological/Medical Applications: For determination of Na, K, Ca and Mg in body fluids and other biological samples. 3. For determination of Alkali and Alkaline Earth Metals in plant Materials, Chemicals, Cements, Oceanography and Ceramic Materials. Get best price at India Mart for spiral diaries: Spiral Diaries - https://m.indiamart.com/impcat/spiral-diaries.html?utm_source=GrowYourkids-il5kh&utm_medium=affiliate&utm_campaign=0624&utm_content=4

  Atomic Absorption Spectrometry is an absorption spectroscopic technique in which radiation of a particular frequency from a source is absorbed by non-excited neutral gaseous atoms  generated in an atomizer in their ground state. The light is absorbed in the UV-visible region and makes transitions to higher electronic energy levels. The amount  of light absorbed is quantified and this amount of absorption helps in determining the analyte concentration. (It follows Beer's Law) The concentration is measured by drawing a calibration curve  after calibrating instrument with a  standard of known concentration. Factors affecting  the amount of light absorbed Length of path transversed Concentration of absorbing atoms in the vapour state. The diagrammatic representation of Atomic Absorption Spectrometer are shown in the below diagram:  Components Used in Atomic Absorbance Spectrometer: 1. Hollow Cathode Lamp: Acts as source of radiation  It is a sha...